Capture, isolation and release of cancer cells with aptamer-functionalized glass bead array
نویسندگان
چکیده
منابع مشابه
Capture, isolation and release of cancer cells with aptamer-functionalized glass bead array.
Early detection and isolation of circulating tumor cells (CTC) can enable better prognosis for cancer patients. A Hele-Shaw device with aptamer functionalized glass beads is designed, modeled, and fabricated to efficiently isolate cancer cells from a cellular mixture. The glass beads are functionalized with anti-epidermal growth factor receptor (EGFR) aptamer and sit in ordered array of pits in...
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Rare circulating tumor cells are a promising biomarker for the detection, diagnosis, and monitoring of cancer. However, it remains a challenge to develop biomedical devices for specific catch and nondestructive release of circulating tumor cells. The purpose of this study was to explore a unique system for cell catch and release by using aptamer-functionalized hydrogels and restriction endonucl...
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Microfluidic-based single cells analysis has been of great interest in recent years, promising disease diagnosis and personalized medicine. Current technologies are challenging in bioselectively isolating specific single cells from complex matrices. Herein, a novel microfluidic platform integrated with cell-recognizable aptamer-encoded microwells was specifically developed to isolate single tum...
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Coated beads retain great importance in the study of cell adhesion and intracellular communication; we present a generally applicable method permitting spatiotemporal control of bead adhesion from cells. Herein we demonstrate in vitro release of a poly-d-lysine (PDL) layer from anionic polystyrene beads, allowing complete bead release from rat cortical neurons post-adhesion.
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The efficient extraction of (bio)molecules from fluid mixtures is vital for applications ranging from target characterization in (bio)chemistry to environmental analysis and biomedical diagnostics. Inspired by biological processes that seamlessly synchronize the capture, transport and release of biomolecules, we designed a robust chemomechanical sorting system capable of the concerted catch and...
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ژورنال
عنوان ژورنال: Lab on a Chip
سال: 2012
ISSN: 1473-0197,1473-0189
DOI: 10.1039/c2lc21251j